Akta purifier user manual

The ge akta purifier 10 is a flexible modular fast protein liquid chromatography system. Akta system training guide school of medicine case. Aktaprime plus user manual 11002644 edition aa 9 1introduction this section is an introduction to aktaprime plus, the documentation included and the content in this manual. Uv, cond, ph w2 mixer b a buffer valve b v6 buffer valve a v5 multiple sample injection air sensor. For further details see akta avant user manual and akta pure user manual references on page 14. There should be a clean titanium filter 18112094 available in the akta supplies drawer that can be swapped out for the one being cleaned. Akta fplc system operators manual marshall scientific. Akta fplc system operators manual marshall scientific by. Our hplc systems such as the agilent 1100 and 1200 series, akta fplcs, and. Data file 11003543 ac process chromatography aktaprocess aktaprocess is an automated liquid chromatography system built for process scaleup and largescale biopharmaceutical manufacturing fig 1. Air accumulates easier in the flow cell if the flow restrictor doesnt generate enough backpressure, hence check that the flow restrictor generates the following back.

Akta fplc explorer 100 operators manual marshall scientific. Shutdown procedure for the akta purifier if you are not planning to continue your experiments the following day, proceed as follows. Box monitor conductivity ph uv detector pumps injector buffers pump a buffer switching pump b valve online filter outlet valve mixer purifier. Systems provide more control than manual purification because of the ability to automatically control the flow rate and monitor the progress of the purification as well as to make controlled gradients and automatically collect fractions. View and download ge akta start maintenance manual online. File containing module user manuals and component instructions for aktafplc equipment pump p920 user manual monitor upc900 user manual. It will help if they get w10 with some good dev support when it comes out. Some of our leading manufacturers include agilent, hp, waters, ge, amersham, dionex and shimazdzu. Aktapurifier 10 cytiva formerly ge healthcare life sciences. Data file 11003543 ac process chromatography aktaprocess. Get familiarized with its use and have its instruction manual handy.

This manual describes the operation of the aktafplc system. Complete ge healthcare akta purifier 10 system from 12007. The proven design has been verified during development and can be userconfigured to meet specific process demands. Belkin f5u220 user manual vvm worked after a soft reset of the device with the sim inserted but only over wifi. Bioscisolutions repairs your akta, biacore, and agilent. Akta avant operating instructions protein production and. Follow the instructions given in the frac920 user manual. Contents monitor upc900 user manual 18112555 edition ag vii. System description, system maintenance and troubleshooting are also. Box900, upc900 monitor, p900 pump, cu950 interface equipped with. All users must read this entire manual to fully understand the safe use of akta. A simple, yet flexible, control software for akta protein purification systems.

Ge akta explorer certified fplc system gmi trusted lab. We offer an assortment of refurbished and used liquid chromatography solutions ranging from hplc, lcms to fplc. Akta pure cytiva, formerly ge healthcare life sciences. Air accumulates easier in the flow cell if the flow restrictor doesnt generate enough backpressure, hence check that the flow restrictor generates the following backpressure. Monitor upc900 user manual 18112555 edition ag vii. Amersham biosciences ab se751 84 uppsala sweden amersham biosciences uk limited amersham place little chalfont. It also contains an overview of the safety instructions. Protein purification the aim of this tutorial is to demonstrate the use of the ubiquitous akta systems and prepacked hitrap chromatography media for rapid, mediumscale protein purification. Loosen the lock knob 3 akta avant user manual 29035184 ae. The akta purifier 100 is perfect for application spanning a wide range of wavelengths the akta purifier 100 allows for rate flows of up to 100 ml per minute fast and easy start to purification work preprogrammed application protocols and method templates speed and reliability for every run fast optimisation and peak identificationaccurate result comparisons and peakfractionation column. Jun 20, 2019 akta purifier manual pdf about this manual. Akta pure 25 purification system is intended for microgramscale protein purifications in tricorn columns up to milligramscale using hitrap and hiscreen columns. Always keep the he inlet above the solution or close the valve.

Flowpath buffervalvea a1 or a2 flowpath buffervalveb. Fraction collector frac950 user manual 181956 edition ae 7 1 introduction 1. Aktafplc optional configurations user manual 18117446 edition aa. Akta systems use intelligent unicorn system control software to combine simplicity with power in protein purification. Unicorn 7 cytiva, formerly ge healthcare life sciences. The platform covers all major chromatographic and cross flow filtration techniques, from the research laboratory to. Aktadesign purification user manual 8 2423 edition ad introduction to this handbook and unicorn 7.

Ge akta explorer amersham pharmacia certified fplc system gmi. Akta avant user manual protein production and purification. July 4, different optional configurations might be akta purifier 10 manual required, this is a modular fast performance liquid chromatography system used primarily for fast separations and purifications of proteins. Mar 29, 2020 akta purifier manual pdf about this manual. Akta chromatography systems have been a staple in protein purification for the past two decades and used throughout the globe. Opening the unicorn software windows for manual control and programmable features purgingpriming pumps and tubing. For fast protein liquid chromatography of tens of grams of protein, akta pure 150 can be used with flow rates up to 150 mlmin. Fraction collector frac950 boyce thompson institute. Gloves and protective eyewear should be worn when handling samples and reagents buffers, however it is preferable that the user remove gloves prior to entering commands via the computer keyboard or mouse. When the user selects a column, the akta explorer instinctively sets the column volume, flow rate and pressure limit parameters into the method template. Apr 19, 2020 akta purifier manual pdf about this manual.

Aktapurifier 10 100 a system pumps mixer 2 4 7 3 1 6 5 2 3 4 5 6 7 1 8 fraction collector frac950 conductivity monitor ph detector inline filter uv cond. Aktaexplorer am ers ha m bio scie nce am er sham bio ci n a m e r s h a m b io s c ie n c a m e r s h a m b io s c ie box n c e monitor conductivity ph uv detector 3 wavelengths pumps column valves injector sample valve buffers phcell electrode sample holder pump a buffer switching valve outlet valve. Purge the pump according to the pump p900 user manual. Please see the aktadesign optimal configuration user manual for the recommended flow rates. Tubing kit changes aktapurifier 100 high flow at delivery 0. Unicorn 7 features an intuitive interface for creating methods by dragging and dropping predefined phases for a wide range of techniques. Full programming control is available for almost any imaginable purification.

It also includes relevant guidance for practical handling and maintenance of instrument components. The aim of this tutorial is to demonstrate the use of the ubiquitous akta systems and prepacked hitrap chromatography media for rapid, mediumscale protein purification. Back pressure increase may indicate treatment is overdue. Manual akta purifier page layout dialog box scribd. This automatic configuration protects the system from damaging events including over pressure.

The protein production laboratories have two machines. Saule saulenhalter injektionsventil inv907 box900 inv907 uv900monitor m925 pumpe p900 p901p903 uvdurchflusszelle mischer m925 anausschalter mischkammer zur abfallflasche. If you manually alter the default values of the column, and thereby exceed the. Manual flowpath b1 or b2 buffervalveb pump a pump b common inlet a2 a1 common inlet b2 b1.

The platform covers all major chromatographic and cross flow filtration techniques, from the research laboratory to process development and manufacturing. Akta labscale protein purification systems are designed for purification of biomolecules, providing speed, performance, and flexibility in research and process development. Amersham biosciences ge fplc system cambridge scientific. Aug 12, 2019 akta purifier manual pdf about this manual. Ge akta purifier user manual download ge akta purifier user manual. All users must read this entire manual to fully understand the safe use. Ge pharmacia akta purifier 100 gmi trusted laboratory. Fplc standard operating procedure akta purifier system page page 1 of 9 fplc standard operating procedure akta system ge note. Akta pure 25 new owners intro the exercise below will give a quick demonstration of how easy and intuitive the akta pure 25 will be for you in demonstrating downstream processing to your students. This high flow rate is necessary when working with. Ge healthcare akta with the ability to purify virtually any biomolecule, akta protein purification systems can handle the simplest and the toughest of challenges. This set up creates a lower flow rate but at a higher pressure required.

Evaluating the uv response calculate the uv response ratios in the following way. Aktaexplorer am ers ha m bio scie nce am er sham bio ci n a m e r s h a m b io s c i en c a m e r s h a m b io s c i e n c e akta system training guide explorer 100 10. Protein purification laboratory of molecular biology. It is intended to be operated as an integrated part of an aktadesign chromatography system running unicorn software. It allows users to speed up process scaleup and cleaning between product. Akta explorer systems include an extensive column library with details of run parameters for each prepacked column. If the leakage persists, replace the assembly according to instructions in the pump p960 user manual. Akta laboratoryscale chromatography systems handbook. Maintenance manually priming buffer inlet lines open draw air out of buffer line go to system control window manual choose. Sections 1 5 contain the necessary information for operating the instrument. Aktapurifier upc 10 cytiva, formerly ge healthcare life. Aktapurifier user guide ge healthcare life sciences manualzz. Check valve is clogged or damaged, causing leakage. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online.